Research Portfolio
Selected projects spanning biofabrication, molecular biotechnology, diagnostics, and analytical workflows.
Developed blood vessel organoids using hiPSCs. The organoids were characterized using histology, immunostaining, bulk RNA seq, and scanning electron microscopy. RNA seq data was analyzed using R studio with packages including Seurat. Images were analyzed using ImageJ and Imaris.
Osteogenic differentiation and characterization workflow
Generated and characterized mineralized spheroids from adipose-derived stem cells (ASCs). Optimized osteogenic culture conditions and performed viability assays (Calcein AM/PI), histology, and fluorescence/confocal microscopy-based analysis.
18S rRNA PCR marker development and sample characterization
Molecular characterization of Fasciola samples from multiple regions of Pakistan. Developed and applied 18S rRNA-based PCR markers for species-specific identification to support diagnostic workflows.
Lahore University of Management Sciences (LUMS)
Conducted master's thesis research focused on molecular cloning, expression, and purification of antigenic regions of Suppressor of Sable and Ballchen proteins from Drosophila melanogaster. Generated polyclonal antibodies against the antigenic region of Ballchen through rabbit immunization and downstream validation. The attached image is of Recombinant plasmid of pET21a-Su(s) construct built in Vector NTI
Mushroom cultivation optimization and caffeine quantification by HPLC
Influence of sample preparation method on the Caffeine Extraction in Coffee. HPLC was used to assess caffeine extraction efficiency from coffee. The attached image is of The chromatogram of coffee sample of 0.4g/40 ml